微信小陈
微信小章
产品简介
购买须知
| 产品名称： | West Nile virus |
|---|---|
| 商品货号： | TS177729 |
| Classification： | Flaviviridae, Flavivirus |
| Agent： | West Nile virus |
| Strain： | Monoclonal antibody to West Nile virus Envelope Protein, Clone E101 (produced in vitro) |
| Common Name： | West Nile virus |
| Applications： | Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E101 hybridoma supernatant by protein G affinity chromatography. The antibody is reported to be reactive using flow cytometry and Western blot analysis. Emerging infectious disease research |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E101 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E101 antibody is reported to bind to the lateral ridge of domain I in the envelope glycoprotein. |
| Product Format： | frozen Each vial of VR-1640 contains approximately 100 µL of purified monoclonal antibody in PBS. |
| Storage Conditions： | xa0Store at -20°C or colder immediately upon arrival Repeated freeze-thaw cycles should be avoided |
| Comments： | Antibody Class: IgG2aκ Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E101 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E101 antibody is reported to bind to the lateral ridge of domain I in the envelope glycoprotein. Functional activity is confirmed by reactivity in immunofluorescence assays using Vero cells (ATCC CCL-81) infected with WNV and by ELISA using WNV-infected cell lysates WNV, Eg101 (Egypt 1951). The antibody is reported to be reactive using flow cytometry and Western blot analysis. |
| Effect on Host： | Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E101 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E101 antibody is reported to bind to the lateral ridge of domain I in the envelope glycoprotein. |
| Name of Depositor： | MS Diamond |
| References： | Oliphant T, et al. Development of a Humanized Monoclonal Antibody with Therapeutic Potential Against West Nile Virus. Nat. Med. 11: 522-530, 2005. PubMed:15852016. Oliphant T, et al. Antibody Recognition and Neutralization Determinants on Domains I and II of West Nile Virus Envelope Protein. J. Virol. 80:12149-12159, 2006. PubMed:17035317. |