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| 产品名称： | pORF2 |
|---|---|
| 商品货号： | TS177801 |
| Designations： | pORF2 |
| Depositors： | NCI-Frederick Cancer Research and Development Center |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli MH3000; MH 3000 |
| Vector Information： | Size (kb): 6.7340002059936520 Vector: pORF2 (plasmid) Promoters: Promoter ompF Construction: pMLB1034, pMLB841 Marker(s):ampR Construct size (kb): 6.734000205993652 Features: marker(s): ampR promoter: ompF replicon: pMB1 terminator: none enhancer: none |
| Applications： | expression vector vector with sequences for protein export |
| Comments： | Expression vector permitting the export of a cloned gene product by formation of a fusion protein with the ompF secretion signal sequence. The lacZ sequences in the vector are out of frame. An insert may alter the reading frame so that a tribred (OmpF-insert-LacZ) protein is produced. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Weinstock GM, et al. Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase. Proc. Natl. Acad. Sci. USA 80: 4432-4436, 1983. PubMed: 6308625 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |