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| 产品名称： | c9186 |
|---|---|
| 商品货号： | TS177812 |
| Designations： | c9186 |
| GenBank Number： | U00027 U00028 U00030 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Vector： | Construct size (kb): 38.29999923706055 |
| Insert： | DNA: genomic Insert lengths(kb): 32.36899948120117 Gene product: NADPH oxidoreductase (old yellow enzyme) ENO2 |
| Insert Size (kb)： | 32.369 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 7.72, 3.00 (vector-associated); 3.92, 3.39, 3.02, 2.28, 2.18, 2.08, 2.08, 1.95, 1.65, 1.52, 1.52, 1.47, 0.86, 0.8, 0.78, 0.76, 0.50, 0.50. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 443830-476199 of the sequence of chromosome VIII. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |