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| 产品名称： | pNO1517 |
|---|---|
| 商品货号： | TS177833 |
| Designations： | pNO1517 |
| Depositors： | D Dean |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.1999998092651370 Vector: pNO1517 (cosmid) Promoters: Promoter str Construction: pBR322, lambda fus3 Marker(s):ampR Construct size (kb): 8.199999809265137 Features: insert detection: rpsL+ marker(s): ampR promoter: str replicon: pMB1 |
| Applications： | vector for constructing genomic libraries vector permitting positive selection for inserts |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. A cosmid vector for positive selection of clones with inserts based on the dominance of the plasmid-coded rpsL+ gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Dean D. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids. Gene 15: 99-102, 1981. PubMed: 6271634 |
| Shipped： | freeze-dried |