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| 产品名称： | pBSL97 |
|---|---|
| 商品货号： | TS177852 |
| Designations： | pBSL97 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.3000001907348630 Vector: pBSL97 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Marker(s):ampR,kanR Construct size (kb): 4.300000190734863 Features: marker(s): ampR promoter for in vitro transcription: T7 (phi10) replicon: f1 replicon: pMB1 MCS: KpnI...SacI MluI MCS: MluI SacI...KpnI coding sequence: nptII (kanR) |
| Applications： | contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.6, 1.3; EcoRI--2.75, 1.4; KpnI--2.5, 1.45; MluI--2.85, 1.25; SstI--2.85, 1.25. Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA. SphI and PstI sites in the nptII gene were removed by site-directed mutagenesis. Contains a nptII (kanR) cassette flanked by multiple cloning sites duplicated in inverted orientation. pBSL97 (TS177852) and pBSL99 (ATCC 87141) differ in the orientations of the multiple cloning sites. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718 |
| Shipped： | freeze-dried |