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微信小章| 产品名称： | REC-1 |
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| 商品货号： | TS177873 |
| Organism： | Homo sapiens, human |
| Tissue： | lymph node |
| Cell Type： | lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Mantle cell lymphoma (B cell non-Hodgkins lymphoma) |
| Age： | 57 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a near-diploid cell line of male origin with a modal chromosome number of 45 and a polyploidy rate of 10%. The karyotype is highly rearranged with most of the cells containing the t(11;14)(q13;q32) common to mantle cell lymphoma. Also, there are approximately 5-6 derivative chromosomes in the karyology that have been described by others. Note: Cytogenetic information is based on initial seed stock at ATCC. |
| Images： |  |
| Derivation： | The human B-cell line Rec-1 was established from a t(11;14)(q13;q32) bearing lymphoma. Ref   Rimokh R, et al. Detection of the chromosomal translocation t(11;14) by polymerase chain reaction in mantle cell lymphomas. Blood: 83(7):1871-1875, 1994. PubMed: 8142653 |
| Clinical Data： | male |
| Antigen Expression： | CD3-, CD5-, CD10-, CD19+, CD20+, CD23-, FMC7- (verified at ATCC) |
| Oncogene： | p53 |
| Genes Expressed： | p53,CD3-, CD5-, CD10-, CD19+, CD20+, CD23-, FMC7- (verified at ATCC) |
| Comments： | Cyclin D1 is overexpressed. Ref Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365xa0An ongoing level of chromosome rearrangements is observed. Ref Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL. Maintain cultures at a cell concentration between 3 X 105 and 1 X 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. xa0 Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.
Interval: Subculture when cell concentration is between 3x105 and 1x106 cells/mL.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Freeze medium: fetal bovine serum, 60%; RPMI-1640 Medium, 30%; DMSO, 10% |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10 D16S539: 11 D5S818: 12,13 D7S820: 10,11 THO1: 9,9.3 TPOX: 8,9 vWA: 17 |
| Population Doubling Time： | approximately 38 hours |
| Name of Depositor： | E. Campo |
| Year of Origin： | 2003 |
| References： | Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697 Rimokh R, et al. Detection of the chromosomal translocation t(11;14) by polymerase chain reaction in mantle cell lymphomas. Blood: 83(7):1871-1875, 1994. PubMed: 8142653 Pinyol M, et al. Inactivation of RB1 in mantle-cell lymphoma detected by nonsense-mediated mRNA decay pathway inhibition and microarray analysis. Blood: 109(12):5422-5429, 2007. PubMed: 17332242 Tagawa H, et al. Genome-wide array-based CGH for mantle-cell lymphoma: identification of homozygous deletions of the proapoptotic gene BIM. Oncogene, 1-11, 2004 Drexler HG, MacLeod RA. Malignant hematopoietic cell lines: in vitro models for the study of mantle cell lymphoma. Leuk. Res. 26: 787-787, 2002. PubMed: 12127550 REC-1 Product Information, DSMZ (German Collection of Microorganisms and Cell Cultures). Retreived July 09, 2007, from http://www.dsmz.de/index.htm. |