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| 产品名称： | pcDNA3L-His-CAMYEL plasmid in Escherichia coli |
|---|---|
| 商品货号： | TS177979 |
| Designations： | pcDNA3L-His-CAMYEL plasmid in Escherichia coli |
| Depositors： | L Jiang |
| Biosafety Level： | 1 |
| Host： | Escherichia coli Stbl2 |
| Vector Information： | Size (kb): 9.38
Vector: pcDNA3L-His-CAMYEL plasmid in E. coli (plasmid)
Type: expression
Promoters: Promoter for expression CMV
Marker(s): neoR, AmpR
Features: primer site: T7 transcription terminator: bGHpA |
| Comments： | A mammalian expression vector that includes CAMYEL (cAMP sensor using YFP-Epac-RLuc), a bioluminescence resonance energy transfer (BRET) sensor.
Vector features: CMV promoter: 232-819; 6xHis: 932-949; CAMYEL: 1025-4927; bGH pA: 4978-5202; SV40 promoter: 5681-6050; Neomycin resistance: 6086-6880; SV40 pA: 7054-7184 ; Amp resistance: 8385-9245 More information on this construct may be found at the AfCS website: www.signaling-gateway.org/data/plasmid/ |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Jiang LI, et al. Use of cAMP BRET sensor to characterize a novel regulation of cAMP by sphingosine-1-phosphate/G13 pathway. J. Biol. Chem. 282: 10576-10584, 2007. |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of E. coli containing the plasmid |