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Nuclear polyhedrosis virus, Autographa californica

货号 TS178084
中文名称 null
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产品名称: Nuclear polyhedrosis virus, Autographa californica
商品货号: TS178084
Classification: Baculovirus
Deposited As: Nuclear pollyhedrosis virus, Autographa californica (Recombinant)
Agent: Nuclear polyhedrosis virus, Autographa californica
Strain: AcCC1
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation:
Nuclear polyhedrosis virus, Autographa californica recombinant with human alpha-galactosidase A precursor gene
Product Format: frozen
Storage Conditions: -70°C or colder
Disclosure: This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments:
AcCC1 and AcCC2 are plaque purified occlusion negative recombinant baculovirus resulting from the co-transfection of Sf-9 cells with pCC4 (transfer vector) and AcMNPV DNA.
Effect on Host:
Expanded nuclei in enlarged cells (25 - 50% increase in diameter). Lysis at late times p.i
Recommended Host:
Sf9 cells (ATCC CRL-1711)
many Lepidopterm insect cell lines
Growth Conditions:
Temperature: 27°C
Incubation: 5-7 days
Cryopreservation:
Storage temperature: -70C
Name of Depositor: DH Calhoun
U.S. Patent:
U.S. Patent Number:
Source:
Nuclear polyhedrosis virus, Autographa californica recombinant with human alpha-galactosidase A precursor gene
References:

Calhoun DH, Coppola G. Recombinant alpha-galactosidase A therapy for Fabry disease. US Patent 5,179,023 dated Jan 12 1993