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微信小章| 产品名称： | palpha2USGII palpha2uSGIIcDNA |
|---|---|
| 商品货号： | TS178091 |
| Designations： | palpha2USGII palpha2uSGIIcDNA |
| GenBank Number： | J00738 |
| Species： | Rattus norvegicus, rat |
| Depositors： | P Feigelson |
| Vector： | Construct size (kb): 5.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.0 Tissue: submaxillary gland Gene product: alpha 2u globulin A2UG* |
| Insert Size (kb)： | 1.0 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following restriction sites (nucleotide position relative to published sequence): ClaI--250; EcoRI--263; HindIII--914; SacI--472; StuI--612. Restriction digests of the clone give the following sizes (kb): PstI--4.4, 1.0; EcoRI--3.95, 1.48; HindIII--4.5, 0.9; BglI--2.95, 2.35; SacI--5.4, ClaI--4.0, 1.5; ClaI+HindIII--4.0, 0.9, 0.65; SstI+HindIII--4.2, 0.9, 0.45. The insert contains the complete cDNA with the coding region extending from 59 to 601. There is a signal peptide of 19 aa. The introns are positioned after the following nucleotides: I--157; II--291; III--365; IV--476; V--578; VI--624. |
| References： | Laperche Y, et al. Tissue-specific control of alpha2u globulin gene expression: constitutive synthesis in the submaxillary gland. Cell 32: 453-460, 1983. PubMed: 6186396 Sarkar P, Feigelson P. Developmentally regulated male-specific transfactor(s) enable in vitro transcription of a cloned alpha 2u-globulin gene. Mol. Endocrinol. 3: 342-348, 1989. PubMed: 2469008 Choy HA, et al. Nuclear factors from expressing tissues interact in vitro with a rat alpha-2u globulin gene intron. J. Biol. Chem. 264: 1754-1759, 1989. PubMed: 2463987 |