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| 产品名称： | pUC19Sce pUCSce |
|---|---|
| 商品货号： | TS178116 |
| Designations： | pUC19Sce pUCSce |
| Depositors： | HP Schweizer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.6860001087188720 Vector: pUC19Sce (plasmid) Construction: pUC19 Marker(s):ampR Construct size (kb): 2.686000108718872 Features: marker(s): ampR replicon: pMB1 restriction site: I-SceI restriction site: SmaI coding sequence: lacZalpha |
| Applications： | vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--2.7; AvaI--2.7; BglI--1.6, 1.1. Useful for rescuing cloned bacterial inserts by digestion with I-SceI, an enzyme recognizing a rare 18 bp sequence which has yet to be found in bacterial DNA. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Schweizer HP, Hoang TT. An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa. Gene 158: 15-22, 1995. PubMed: 7789804 |
| Shipped： | freeze-dried |