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购买须知
| 产品名称： | plambda500 p500 |
|---|---|
| 商品货号： | TS178252 |
| Designations： | plambda500 p500 |
| Species： | Homo sapiens, human |
| Depositors： | C Caskey, PI Patel |
| Vector： | Construct size (kb): 10.25 |
| Insert： | DNA: genomic Insert lengths(kb): 5.900000095367432 Gene product: hypoxanthine phosphoribosyltransferase pseudogene 2 HPRTP2 Alleles: A1, A2 |
| Insert Size (kb)： | 5.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.1 kb doublet; HindIII--10.25; PstI--10.25; EcoRI/BamHI--5.6, 4.1, 1.25; EcoRI/HindIII--5.1, 4.4, 0.9. The 0.9 kb EcoRI/HindIII fragment represents the HPRTP2 gene. The insert includes a 5.0 kb EcoRI fragment and a 0.9 kb EcoRI/HindIII fragment, with the latter subcloned from Hulambda5. The 3.8 kb constant band is from the X chromosome. Enzyme(s) not detecting polymorphism: TaqI, PstI, BstNI, XbaI, SacI, PvuII, HindIII, HincII, StuII, MboI, EcoRV. |
| References： | Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192 Patel PI, et al. Organization of the HPRT gene and related sequences in the human genome. Somatic Cell Mol. Genet. 10: 483-493, 1984. PubMed: 6089358 C. Thomas Caskey, personal communication |