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| 产品名称： | pCWA274 |
|---|---|
| 商品货号： | TS178267 |
| Designations： | pCWA274 |
| GenBank Number： | L27425 |
| Species： | Homo sapiens, human |
| Depositors： | CW Anderson |
| Vector： | Construct size (kb): 10.35000038146973 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript KS+ Intact vector size: 2.964 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: DNA-PK(CS) Gene name: DNA-activated serine/threonine protein kinase (catalytic subunit) Contains complete coding sequence?: N Chromosome: 8; Localization: 8 q11 Type of DNA: genomic Insert end: EcoRI Insert end: EcoRI Insert size (kb): 7.392 Cross references: DNA Seq. Acc.: L27425 Nucleotides 1-7393 of the insert correspond to nucleotides 247-7693 of L27425. Insert lengths(kb): 7.392000198364258 Gene product: DNA-activated serine/threonine protein kinase (catalytic subunit) DNA-PK(CS) Alleles: -, + |
| Insert Size (kb)： | 7.392 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.4, 3.0; BamHI--10.4; BglII--9.3, 1.05; HindIII--6.0, 4.4; SmaI--8.0, 2.4. The insert contains the following restriction sites (approximate kb from the 5 end): SacI--3.06, 5.68; BglII--5.15, 6.00; SmaI--5.26; HindIII--6.01. PvuII polymorphism was detected in the PCR product generated with upstream primer (nt 6229-6252 of L27425) and downstream primer (compliment of nt 7570-7589). |
| References： | Sipley JD, et al. Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8. Proc. Natl. Acad. Sci. USA 92: 7515-7519, 1995. PubMed: 7638222 |