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| 产品名称： | pCMV-SCAP |
|---|---|
| 商品货号： | TS178306 |
| Designations： | pCMV-SCAP |
| GenBank Number： | U67060 |
| Depositors： | JL Goldstein, University of Texas Southwestern Medical Center |
| Applications： | In another host, produces protein SREBP cleavage-activating protein |
| Vector： | Name of construct: pCMV-SCAP Excise insert: AseI + XbaI Promoter giving sense strand: CMV Size of construct (kb): 10.4 Markers: ampR, G418R Name of vector: pRc/CMV7SB Intact vector size: 6.2 Type of vector: phagemid Vector end: SalI Vector end: XbaI Cloning sites: SalI notI Polylinker sites: SalI KpnI SacI BamHI EcoRI PstI EcoRV NotI XhoI AvaI XbaI SacI Host range: Escherichia coli, mammalian cells Features (with orientation and position when available): promoter for expression: CMV, → other: adenovirus/immunoglobulin hybrid intron, → MCS: SalI...SacI, → terminator: BGH polyadenylation, → replicon: f1 promoter: SV40, → replicon: SV40 marker(s): neoR, → terminator: SV40 polyadenylation, → replicon: f1 marker(s): ampR, → |
| Insert： | Genome: hamster Gene symbol: SCAP* Gene name: SREBP cleavage-activating protein Contains complete coding sequence?: Y Type of DNA: cDNA Insert size (kb): 4.2 Cross reference: DNA seq. Acc.: U67060 |
| Insert Size (kb)： | 4.20 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): AseI/XbaI--4.5, 2.4, 1.4, 1.2, 0.64, 0.25; BamHI--8.1, 2.3; EcoRI--5.0 (doublet), 0.39. |
| References： | Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195 |