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| 产品名称： | lambdaSP11 |
|---|---|
| 商品货号： | TS178337 |
| Designations： | lambdaSP11 |
| GenBank Number： | M12950 |
| Species： | Homo sapiens, human |
| Depositors： | M Lefranc |
| Vector： | Construct size (kb): 44.70000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 15.19999980926514 Tissue: T-lymphocyte SP-F7 cell line Gene product: T cell receptor, gamma, joining segment JP1 TCRGC1 |
| Insert Size (kb)： | 15.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--19.0, 12.0, 6.0, 4.8, 2.5; EcoRI--20.0, 10.5, 5.4, 2.25, 1.9, 1.6, 1.25, 1.1, 0.8; BamHI--20.0, 12.0, 9.0, 2.9; HindIII--22.0, 12.0, 3.7, 2.9, 2.2, 1.6, 1.4; PvuI--20.0, 15.0, 8.0; SacI--20.0, 9.5, 6.4, 4.2, 1.8, 1.1, 0.8. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--1.1, 3.3, 4.2, 5.6, 7.4, 9.0, 14.1, 15.0; KpnI--1.3, 10.0; HindIII--2.8, 4.2, 6.5, 8.2, 10.5, 13.3; BamHI--12.5; XhoI--8.0. Contains a functional rearrangement of V2 (subgroup I) and JP1. A portion of the EcoRI/HindIII fragment (1.1-2.8 kb from the 5 end) was sequenced to demonstrate a join through a N-diversity region. Also contains germline JP, germline J1 and exon 1 of C1. |
| References： | Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738 |