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| 产品名称： | B144 |
|---|---|
| 商品货号： | TS178391 |
| Designations： | B144 |
| Species： | Mus musculus, mouse |
| Depositors： | F Shen |
| Vector： | Construct size (kb): 3.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.4000000059604645 Tissue: B lymphocyte, I.29 cell line Gene product: DNA segment, Chr 17, Tampa Bay D17Tbr1* |
| Insert Size (kb)： | 0.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | A BamHI digest will give a 0.6 kb fragment containing the insert, and a BamHI/PstI double digest will give a 0.55 kb fragment containing the insert. Insert contains the following sites separated by (bp)(approximate): PstI- 155- HpaII- 80- PvuII- 20- EcoRV- 135- BamHI. Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 0.6; XhoI--2.9, 0.72; SalI--3.5; BglI--2.4, 1.15; PstI--2.19, 1.25. Contains the 3 half of a cDNA from an unknown gene in the H-2S:H-2D interval of mouse chromosome 17. The I.29 cell line was derived from a spontaneous B-cell ascites tumor that originated in an I/St/Boy mouse. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Tsuge I, et al. A gene in the H-2S:H-2D interval of the major histocompatibility complex which is transcribed in B cells and macrophages. Immunogenetics 26: 378-380, 1987. PubMed: 3117682 Fung-Win Shen, personal communication |