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| 产品名称： | pCu414CUP1 |
|---|---|
| 商品货号： | TS178546 |
| Designations： | pCu414CUP1 |
| Depositors： | S Labbe, DJ Thiele |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.2699999809265140 Vector: pCu414CUP1 (phagemid) Promoters: Promoter for expression CUP1 Construction: pRS414, CUP1 promoter Marker(s):TRP1,ampR Construct size (kb): 5.269999980926514 Features: marker(s): TRP1 marker(s): ampR promoter for expression: CUP1 replicon: ARSH4 replicon: f1 replicon: pMB1 MCS: SpeI...XhoI terminator: CYC1 centromere: CEN6 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the vector give the following sizes (kb): SacI--5.3; SacI/SpeI--5.0, 0.26. Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae. |
| References： | Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452 |
| Related Products： | component of:ATCC 87778 |