| 产品名称: | lambdaPM-4783 4783 |
|---|---|
| 商品货号: | TS178572 |
| Designations: | lambdaPM-4783 4783 |
| Species: | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors: | MV Olson, L Riles |
| Vector: | Construct size (kb): 0.0 |
| Insert: | DNA: genomic Gene product: pyruvate dehydrogenase, E1 alpha polypeptide 1(pyruvate dehydrogenase E1-alpha subunit) BMH1 |
| Related Products: | component of:ATCC 77284 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.356, 1.767, 0.995, 2.649, 0.851, 1.097, 0.679, 2.468, 1.851, 1.024, 0.867, 0.854, 1.689, 2.785. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.68, 0.85, 0.98, 1.0, 1.1, 1.4. Overlaps clones with ATCC number(s): 70106, 70691, 70881. PDA1 lies almost entirely in the 1.7 kb HindIII fragment common to clones lambdaPM-3058 (ATCC 70106) and lambdaPM-4783 (TS178572). DMC1 is in the 2.9 kb HindIII/EcoRI fragment common to clones lambdaPM-3058 (ATCC 70106) and lambdaPM-4783 (TS178572). |
| Classification: | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References: | Olson MV, et al. Random-clone strategy for genomic restriction mapping in yeast. Proc. Natl. Acad. Sci. USA 83: 7826-7830, 1986. PubMed: 3463999 Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 Wenzel TJ, et al. Promoter analysis of the PDA1 gene encoding the E1alpha subunit of the pyruvate dehydrogenase complex from Saccharomyces cerevisiae. Yeast 10: 297-308, 1994. PubMed: 8017100 |