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| 产品名称： | pLmTIM |
|---|---|
| 商品货号： | TS178598 |
| Designations： | pLmTIM |
| GenBank Number： | X74797 |
| Species： | Leishmania mexicana (Biagi) Garnham |
| Depositors： | L Kohl |
| Vector： | Construct size (kb): 5.349999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 0.75 Gene product: triose phosphate isomerase tim |
| Insert Size (kb)： | 0.75 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.4; NdeI--5.4; BamHI/NdeI--4.6, 0.75; SphI--4.7, 0.65; PvuI--1.55, 1.35, 1.1, 1.1, 0.2. The insert contains the following restriction sites (approximate kb from the 5 end): PvuI--0.2, 0.45; SphI--0.35; PstI--0.39; EcoRV--0.68. Constructed by amplification from genomic DNA using the following primers that introduce, respectively, NdeI and BamHI sites: sense 5-GCGCCCATATGTCCGCCAATC-3 and antisense 5-GCTATAATTAGTTTGGATCCATCG-3. |
| References： | Kohl L, et al. Triose-phosphate isomerase of Leishmania mexicana mexicana. Cloning and characterization of the gene, overexpression in Escherichia coli and analysis of the protein. Eur. J. Biochem. 220: 331-338, 1994. PubMed: 8125090 |