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| 产品名称： | Human echovirus 1 |
|---|---|
| 商品货号： | TS178746 |
| Classification： | Picornaviridae, Enterovirus, Enterovirus B |
| Agent： | Human echovirus 1 |
| Strain： | Farouk |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | This item has been treated with diethyl ether to remove Mycoplasma at the ATCC. The previous item number was VR-31™
Monkeys and rabbits yield satisfactory immune sera.xa0 No apparent infection in monkeys, rabbits or newborn mice.xa0 Cross-reacts antigenically with Echovirus 8. |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | LLC-MK2 cells (ATCC® CCL-7.1™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | JL Melnick, Yale University School of Medicine |
| Source： | Feces from normal infant, Egypt, 1951 |
| Year of Origin： | 1951 |
| References： | Melnick JL, Agren K. Poliomyelitis and Coxsackie viruses isolated from normal infants in Egypt. Proc. Soc. Exp. Biol. Med. 81: 621-624, 1952. PubMed: 13037743 |