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| 产品名称： | pGH205 |
|---|---|
| 商品货号： | TS178757 |
| Designations： | pGH205 |
| Depositors： | OmniGene, Inc., S Guterman, OmniGene, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Streptomyces lividans |
| Vector Information： | Size (kb): 12.1999998092651400 Vector: pGH205 (plasmid) Promoters: Promoter aph Construction: pGH202 pGH8 Marker(s):ampR tsrR Construct size (kb): 12.19999980926514 Features: marker(s): ampR marker(s): tsrR promoter: aph replicon: pIJ101 replicon: pMB1 signal peptide coding region: endo H coding sequence: endo H |
| Applications： | produces protein endo-beta-N-acetylglucosaminidase shuttle vector vector with sequences for protein export endo-beta-N-acetylglucosaminidase |
| Comments： | Shuttle expression vector permitting secretion of fusion proteins from S. lividans hosts. pGH202 (ATCC 39896) and pGH205 (TS178757) differ in that the latter includes a promoter element for aminoglycoside phosphotransferase (aph). S. lividans transformed with this plasmid will secrete endo H when grown in this medium: Difco yeast extract (3 g/L), Difco peptone (5 g/L), Oxoid malt extract (3 g/L), glucose (10 g/L), sucrose (340 g/L), and 5 mM MgCl2. |
| Media： | YEME-thiostrepton medium
|
| Growth Conditions： | Temperature: 30.0°C |
| References： | Guterman S. Streptomyces secretion vector. US Patent 4,745,056 dated May 17 1988 Robbins PW, et al. Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosamine. J. Biol. Chem. 259: 7577-7583, 1984. PubMed: 6429133 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |