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| 产品名称： | pGPTV-BAR |
|---|---|
| 商品货号： | TS178778 |
| Designations： | pGPTV-BAR |
| Depositors： | R Masterson, D Becker |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 13.4000005722045900 DESCRIPTION OF VECTOR: Intact vector size: 13.400 Type of vector: plasmid Cloning sites: EcoRI SstI SmaI XbaI HindIII BamHI Polylinker sites: Construction: pBIN19, pBI101, pBIB-HYG Host range: Rhizobium radiobacter; higher plants; Escherichia coli Features (with orientation and position when available): marker(s): kanR replicon: RK2 Vector: pGPTV-BAR (plasmid) Construction: pBIN19, pBI101, pBIB-HYG Marker(s):kanR Construct size (kb): 13.40000057220459 Features: marker(s): kanR replicon: RK2 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--13.0; BglII--8.4, 3.1, 1.4, 0.56; BamHI--13.0. Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI--1.2; EcoRI--11.2; SstI--11.5. Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced. The order of the major features of this plasmid is: EcoRI - pAnos - SstI - uidA - SmaI - XbaI - HindIII - Pnos - BglII - bar - BglII - BamHI - pAg7 - T-DNA - BglII - RK2 ori - kanR. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Becker D, et al. New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol. Biol. 20: 1195-1197, 1992. PubMed: 1463855 |
| Shipped： | frozen |
| Shipping Information： | Distributed: frozen |