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微信小章| 产品名称： | Kluyveromyces lactis (Dombrowski) van der Walt |
|---|---|
| 商品货号： | TS178801 |
| Strain Designations： | 61, type C, CBS 2359, CCRC 21716, DSM 70799, IFO 1267, NRRL Y-1140, VKPM Y-1174 |
| Application： | Degrades whey permeate
Hydrolyzes whey lactose
Produces L-methionine
Produces beta-galactosidase beta-D-galactosidase
Produces killer toxin
Produces L-methionine from whey permeate |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Genotype： | a k1+ k2+ |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Mating Type： | Mates with ATCC® 8651™ |
| Ploidy： | haploid |
| Comments： | Regulation of pyruvate dehydrogenase activity by E1alpha subunit levels
Resistant to killer toxin produced by Schwanniomyces occidentalisxa0(ATCC® 44252™)
Carries two linear DNA plasmids, pGK11 and pGK12
Transcriptional regulation of beta-galactosidase gene
Effect of lactose permeabilization on beta-galactosidase production
Nonflocculating
Killer, immune
Genome sequencing strain (Genolevures Consortium, France).
Sequenced mitochondrial genome. |
| Medium： | ATCC® Medium 200: YM agar or YM broth ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence AAGGATCATTAAAGATTATGAATGAGTAGATTACTGGGGGAATCGCTGAATATGGCCTGCGCTTAATTGCGCGGCTAATTCTTGATTTTCTGCTATCAGTTTTCTTTCTCTCATCCTAAACACAATGGAGTTTTTTCTCTATGAACTACTTCCCTGGAGAGCTCGTCTCTCCAGTGGACATAAACACAAACAACATTTTGCATTATGAAAAACTATTTATCAAGAAATTTAATATTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAATTGCGATATGTATTGTGAATTGCAGATTTTCGTGAATCATCAAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCTTTGGGTTTGGTAGTGAGTGATACTCGTTTTTCGGGTTAACTTGAAAGTGGCTAGCCGTTGCCTTCTGCGTGAGCAGGGCTGCGTGTCAAGTCTATGGACTCGACTCTTGCACATCTACGTCTTAGGTTTGCGCCAATTCGTGGTAAGCTAGGGTCAATGAGCTTATAGGTGTTATAAAGACTCGCTGGTGTTTGTCTCCTTGAGGCATACGGCTTAATCAAAACTCTCAAAGTTTGACCTCAAATCAGGTAGGAATACCCGCTGAACTTAAG D1D2 region of the 26S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTTGAAGAAGGCAACTTTGTAGCTGGTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGATCCCAGTTATTTGTAAAGTGCTTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGCGTTTGCTTCGGCTTTCGCTGGGCCAGCATCAGTTTTAGCGGTTGGATAAATCCTCGGGAATGTGGCTCTGCTTCGGTAGAGTGTTATAGCCCGTGGGAATACAGCCAGCTGGGACTGAGGATTGCGACTTTTGTCAAGGATGCTGGCGTAATGGTTAAATGCCG |
| Name of Depositor： | Lederle Labs. |
| Chain of Custody： | ATCC <-- Lederle Labs. <-- N.C. Laffer 61, type C (Zygosaccharomyces lactis) |
| Isolation： | Creamery, USA |
| Cross References： | Nucleotide (GenBank) : KU729077 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KU729173 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : X72499 nucleotide sequence of KlGGS1 gene Nucleotide (GenBank) : X81421 K.lactis gene for alpha-alpha-trehalose 1-D-glucohydrolase. Nucleotide (GenBank) : CR382121 Kluyveromyces lactis strain NRRL Y-1140 chromosome A complete sequence. |
| References： | Gunge N, et al. Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. J. Bacteriol. 145: 382-390, 1981. PubMed: 6257636 Wickerham LJ, Burton KA. Occurrence of yeast mating types in nature1,3. J. Bacteriol. 63: 449-451, 1952. PubMed: 14938317 Lacy LR, Dickson RC. Transcriptional regulation of the Kluyveromyces lactis beta- galactosidase gene. Mol. Cell. Biol. 1: 629-634, 1981. PubMed: 9279376 . . Biotechnol. Tech. 6: 289-292, 1992. Schaffrath R, Meacock PA. Kluyveromyces lactis killer plasmid pGKL2: molecular analysis of an essential gene, ORF5. Yeast 11: 615-628, 1995. PubMed: 7483835 Bellal M, et al. Structural differences in parietal peptidomannans from flocculating strains of Kluyveromyces lactis. Can. J. Microbiol. 41: 323-335, 1995. Luyten K, et al. Disruption of the Kluyveromyces lactis GGS1 gene causes inability to grow on glucose and fructose and is suppressed by mutations that reduce sugar uptake. Eur. J. Biochem. 217: 701-713, 1993. PubMed: 8223613 Dickson RC, Markin JS. Physiological studies of beta-galactosidase induction in Kluyveromyces lactis. J. Bacteriol. 142: 777-785, 1980. PubMed: 6769910 Siso MI. Beta-galactosidase production by Kluyveromyces lactis on milk whey: batch versus fed-batch cultures. Process Biochem. 29: 565-568, 1994. Bellal M, et al. Flocculation dispersion in Kluyveromyces lactis. Process Biochem. 30: 641-648, 1995. . . Enzyme Microb. Technol. 16: 303-310, 1994. Butler AR, et al. Analysis of the response of Saccharomyces cerevisiae cells to Kluyveromyces lactis toxin. J. Gen. Microbiol. 137: 1749-1757, 1991. PubMed: 1955863 Zeeman AM, et al. Regulation of pyruvate metabolism in chemostat cultures of Kluyveromyces lactis CBS 2359. Yeast 16: 611-620, 2000. PubMed: 10806423 Chen WB, et al. Isolation, purification, and characterization of a killer protein from Schwanniomyces occidentalis. Appl. Environ. Microbiol. 66: 5348-5352, 2000. PubMed: 11097913 Kitamoto HK, Nakahara T. Isolation of an L-methionine-enriched mutant of Kluyveromyces lactis grown on whey permeate. Process Biochem. 29: 127-131, 1994. Dujon B, et al. Genome evolution in yeasts. Nature 430: 35-44, 2004. Souciet JL, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 1. A set of yeast species for molecular evolution studies. FEBS Lett. 487: 3-12, 2000. Bolotin-Fukuhara M, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 11. Kluyveromyces lactis. FEBS Lett. 487: 66-70, 2000. Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262 Zivanovic Y, et al. Complete nucleotide sequence of the mitochondrial DNA from Kluyveromyces lactis. FEMS Yeast Res. 5: 315-322, 2005. PubMed: 15691736 |