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| 产品名称： | pcCSF-17 CMCC 2347 |
|---|---|
| 商品货号： | TS178822 |
| Designations： | pcCSF-17 CMCC 2347 |
| Species： | Homo sapiens, human |
| Depositors： | Cetus Corp. |
| Applications： | in another host, produces protein colony stimulating factor 1 (macrophage) |
| Vector： | Construct size (kb): 4.80 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pcD Intact vector size: 3.000 Type of vector: plasmid Cloning sites: XhoI BamHI Polylinker sites: Construction: pBR322, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): enhancer: SV40 marker(s): ampR promoter: SV40 early, late replicon: pMB1, SV40 terminator: SV40 late Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CSF1 Genomic copy number: unique Gene name: colony stimulating factor 1 (macrophage) Contains complete coding sequence?: U Chromosome: 1; Localization: 1 p21-p13 Tissue: MIA PaCa-2 cell line Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 1.64 Cross references: Insert lengths(kb): 1.64 Tissue: MIA PaCa-2 cell line Gene product: colony stimulating factor 1 (macrophage) CSF1 Target Gene: colony stimulating factor 1 (macrophage) |
| Insert Size (kb)： | 1.640 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; HindIII--4.8; BamHI--3.0, 0.9, 0.87; PstI--3.0, 1.3, 0.35; SalI--4.8. When this plasmid is transfected into COS cells, the product shows activity by radioreceptor assay, bone marrow proliferation assay, and CFU assay. This insert encodes a mature CSF-1 protein of 224 amino acids. The insert includes approximately 180 bp 5 untranslated sequence and approximately 700 bp 3 untranslated sequence. |
| References： | Kawasaki ES, et al. DNA encoding for CSF-1 and accompanying recombinant systems. US Patent 4,847,201 dated Jul 11 1989 Shadle PJ, et al. Conjugation of polymer to colony stimulating factor-1. US Patent 4,847,325 dated Jul 11 1989 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |