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| 产品名称： | MDR1.1 pGEM3Zf(-)Xba-MDR1.1 in E. coli |
|---|---|
| 商品货号： | TS178901 |
| Designations： | MDR1.1 pGEM3Zf(-)Xba-MDR1.1 in E. coli |
| Species： | Homo sapiens, human |
| Depositors： | P Borst |
| Vector： | Construct size (kb): 7.3 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.1 Tissue: liver Gene product: P glycoprotein 1/multiple drug resistance 1 MDR1 |
| Insert Size (kb)： | 4.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | Purified plasmid DNA: ATCC 65705 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--4.15, 3.15; EcoRI--7.3; HindIII--7.3; BglII--6.3; 1.0; PstI--7.3. According the sequence of GenBank accession M14758, the insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--1.27; HindIII--2.14. The sequence extends from position -98 to +4054 relative to the translation start site, and encodes a glycine at aa 185. A full-length cDNA (coding sequence 3842 nt). When inserted into a eukaryotic expression vector and transfected into BRO melanoma cells, the sequence confers multidrug resistance. Constructed by adding XbaI linkers to the insert from an EcoRI-linkered library, and cloning into a derivative of pGEM-3Zf(-) in which the multiple cloning sequence had been replaced by a single XbaI site. The insert is oriented with the 5 end closer to the T7 RNA polymerase promoter. |
| References： | van der Bliek AM, et al. Sequence of mdr3 cDNA encoding a human P-glycoprotein. Gene 71: 401-411, 1988. PubMed: 2906314 Lincke CR, et al. Multidrug resistance phenotype of human BRO melanoma cells transfected with a wild-type human mdr1 complementary DNA. Cancer Res. 50: 1779-1785, 1990. PubMed: 1968359 Piet Borst, personal communication |