微信小陈
微信小章
产品简介
购买须知
| 产品名称： | TFTA1 3721.95.161.4 |
|---|---|
| 商品货号： | TS178921 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14). |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the A chain (A1 and A2) of the plant toxin ricin |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the A chain (A1 and A2) of the plant toxin ricin |
| Comments： | Animals were immunized with ricin A chain. Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14). The antibody does not cross-react with the ricin B chain. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | R Fulton |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Fulton RJ, et al. Purification of ricin A1, A2, and B chains and characterization of their toxicity. J. Biol. Chem. 261: 5314-5319, 1986. PubMed: 3957927 |