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| 产品名称： | pRB381 |
|---|---|
| 商品货号： | TS178952 |
| Designations： | pRB381 |
| Depositors： | R Bruckner |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.8000001907348630 Vector: pRB381 (plasmid) Construction: pUB110, pBR322, lacZ Marker(s):ampR,bleR,neoR Construct size (kb): 8.800000190734863 Features: insert detection: lac marker(s): ampR, neoR, bleR replicon: pUB110, pMB1 terminator: rrnB, to phage lambd |
| Applications： | shuttle vector vector permitting visual detection of activity in colonies |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/BglI--5.0, 2.2, 1.1, 0.8; BamHI--9.4; BglI/BglII--3.5, 2.2, 2.0, 1.3. Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media. May not be suitable for cloning very strong expression signals. Translational fusion shuttle vector using lacZ as reporter. Also useful for the construction of fusion proteins. Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin. The 5 truncated lacZ gene, lacking transcriptional and translational initiation signals, is useful in both E. coli and in B. subtilis, since the endogenous beta-galactosidase level in B. subtilis is low. The order of the major features in the plasmid is: To terminator - HindIII/MCS/BamHI - lacZ - rrnB terminator - ampR - pMB1 ori - pUB110 ori - neoR - bleR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Bruckner R. A series of shuttle vectors for Bacillus subtilis and Eschericia coli. Gene 122: 187-192, 1992. PubMed: 1452028 |
| Shipped： | freeze-dried |