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| 产品名称： | pCHR81 |
|---|---|
| 商品货号： | TS179029 |
| Designations： | pCHR81 |
| Depositors： | C Sasakawa |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 40.0000000000000000 Vector: pCHR81 (plasmid) Marker(s):kanR,tmpR Construct size (kb): 40.0 Features: marker(s): kanR, tmpR replicon: R388 |
| Applications： | vector useful for transposon mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--32, 4.0, 1.8; PstI--36, 2.7, 1.8; HindIII--38, 3.8, 3.0. Replication is temperature-sensitive. Can be used as a suicide vector for the delivery of transposable elements. Constructed by mutagenizing R388 to construct a temperature sensitive derivative and then transposing on Tn5. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Sasakawa C, Yoshikawa M. A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis. Gene 56: 283-288, 1987. PubMed: 2824292 |
| Shipped： | frozen |