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| 产品名称： | pWE16 |
|---|---|
| 商品货号： | TS179060 |
| Designations： | pWE16 |
| Depositors： | GA Evans |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.8000001907348630 Vector: pWE16 (cosmid) Promoters: Promoter T3 Construction: pCV107, pCV108, pJB8 Marker(s):ampR,mtxR Construct size (kb): 8.800000190734863 Features: marker(s): ampR, mtxR promoter: T3, T7 replicon: pMB1 |
| Applications： | contains easily purifiable cassette(s) for construction expression vector integrating vector shuttle vector vector for constructing genomic libraries vector permitting RNA synthesis in vitro vector permitting positive selection for integration |
| Comments： | pWE15 (ATCC 37503) and pWE16 (TS179060) differ only in the markers selectable in vertebrate cells. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Useful for constructing genomic libraries and chromosome walking and for gene transfer using dihydrofolate reductase as a selectable marker. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Wahl GM, et al. Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer. Proc. Natl. Acad. Sci. USA 84: 2160-2164, 1987. PubMed: 3031668 Evans GA, Wahl GM. Cosmid vectors for genomic walking and rapid restriction mapping. Methods Enzymol. 152: 604-610, 1987. PubMed: 2443810 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |