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| 产品名称： | pYN1738 |
|---|---|
| 商品货号： | TS179104 |
| Designations： | pYN1738 |
| GenBank Number： | K01702 |
| Species： | Gallus gallus, chicken |
| Depositors： | BR Olsen, R Haronen |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.200000047683716 Tissue: sternal cartilage, 17-day-old embryo Gene product: collagen, type IX, alpha 1 COL9A1 |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI--3.3, 1.7, 0.64; PstI--3.7, 1.4, 0.78; HindIII--6.0. Insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.4; BglI--0.6, 0.85, 0.9, 2.1; ClaI--0.3; PstI--0.55, 1.35; PvuII--0.55, 1.7, 1.8, 2.3; SacI--0.2, 0.8, 0.9, 2.7; SmaI--0.4, 1.3, 2.0; XbaI--2.7; XhoI--0.6. Insert lacks the complete 5 end. |
| References： | Ninomiya Y, Olsen BR. Synthesis and characterization of cDNA encoding a cartilage-specific short collagen. Proc. Natl. Acad. Sci. USA 81: 3014-3018, 1984. PubMed: 6328487 Ninomiya Y, et alThe molecular biology of collagens with short triple-helical domainsIn: Ninomiya Y, et alExtracellular matrix genesNew YorkAcademic Presspp. 79-114, 1990 |