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微信小章| 产品名称： | HeLaRC32 HeRC32 |
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| 商品货号： | TS179116 |
| Organism： | Homo sapiens, human |
| Tissue： | cervix |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain human papilloma virus 18 (HPV-18)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 31 |
| Gender： | female |
| Ethnicity： | Black |
| Applications： | These cells generate rAAV preparations with high titers of infectious particles which are essentially free of adenoviruses for biological, preclinical, clinical or pharmaceutical applications. HelaRC32 is a stable packaging cell line expressing the rep and cap genes for recombinant adeno-associated virus type 2 (rAAV-2) assembly which constitutes an attractive alternative to transient transfection protocols. Ref |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | This is a clone of HeLa cells that harbors one to two rep-cap gene copies per cell. |
| Clinical Data： | 31 Black female |
| Genes Expressed： | Rep proteins (Rep 78, Rep 68, Rep 52 and Rep 40), Cap proteins (VP1, VP2 and VP3) |
| Cellular Products： | Rep proteins (Rep 78, Rep 68, Rep 52 and Rep 40) Cap proteins (VP1, VP2 and VP3) |
| Comments： | This is a clone of HeLa cells that harbors one to two rep-cap gene copies per cell. Upon vector transfection and adenovirus infection, efficient rAAV assembly correlated with a 100-fold amplification of the integrated rep-cap sequence with the inverted terminal repeats (ITRs) deleted. xa0 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended.
Medium renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Dulbeccos Modified Eagles Medium, 70%; fetal bovine serum, 20%; DMSO, 10% |
| STR Profile： | D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 THO1: 7 TPOX: 8, 12 CSF1PO: 9, 10 Amelogenin: X |
| Name of Depositor： | P Moullier |
| References： | Chadeuf G, et al. Efficient recombinant adeno-associated virus production by a stable rep-cap HeLa cell line correlates with adenovirus-induced amplification of the integrated rep-cap genome. J. Gene Med. 2(4):260-268, 2000. PubMed: 10953917. Tessier J, et al. Characterization of adenovirus-induced inverted terminal repeat-independent amplification of integrated adeno-associated virus rep-cap sequences. J. Virol. 75(1):375-383, 2001. PubMed: 11119606 |
Tessier J, et al. Characterization of adenovirus-induced inverted terminal repeat-independent amplification of integrated adeno-associated virus rep-cap sequences. J. Virol. 75(1):375-383, 2001. PubMed: 11119606