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| 产品名称： | pSGP572a |
|---|---|
| 商品货号： | TS179128 |
| Designations： | pSGP572a |
| Depositors： | J Hodson |
| Biosafety Level： | 1 |
| Host： | Host range: Schizosaccharomyces pombe; Escherichia coli |
| Vector Information： | Construct size (kb): 9.3 |
| Applications： | shuttle vector vector permitting construction of fusion proteins |
| Comments： | This Schizosaccharomyces pombe expression vector is useful for constructing fusion proteins with a C-terminal GFP tag. Promoter is nmt. GFP sequence corresponds to Genbank Accession number U87624 with nucleotides 32-747 of GFP matching nucleotides 21-736 of U87624, with additional modification S65T by changing codon to ACC. The order of the major feature in the plasmid is: nmt promoter - XhoI - BglII - SacII - NotI - TTG - GFP - TAG - SalI - SmaI - termination sequence - arsI - pUC119 (3.3 kb) - ura4. Restriction digests of the vector gave the following bands (in kb): EcoRI - 8.1, 1.2; SacI - 9.3; XhoI/SmaI - 8.5, 0.75. -- ATCC staff |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |