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| 产品名称： | pMCT128.1 MCT128.1 |
|---|---|
| 商品货号： | TS179200 |
| Designations： | pMCT128.1 MCT128.1 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Otherwise, RFLPs were observed under normal hybridization and wash conditions. |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: genomic Insert lengths(kb): 3.0 Gene product: DNA Segment, single copy D11S144 Alleles: A1, A1, A1, A2, A2, A2 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.7, 1.2, 0.94, 0.74; PstI--3.6, 1.7, 0.6; EcoRI/HindIII--3.0, 1.7, 0.82, 0.5; EcoRI--4.25, 0.87, 0.43. This probe was also known as D11S285. Codominant segregation demonstrated in 46 3-generation families. This probe was pre-associated with excess human DNA before hybridization. Otherwise, RFLPs were observed under normal hybridization and wash conditions. Enzyme(s) not detecting polymorphism: BglII, PvuII, TaqI. |
| References： | Smith M, et al. Mapping of a gene determining tuberous sclerosis to human chromosome 11q14-11q23. Genomics 6: 105-114, 1990. PubMed: 2303253 Charmley P, et al. A primary linkage map of the human chromosome 11q22-23 region. Genomics 6: 316-323, 1990. PubMed: 2307473 Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence MCT128.1 on chromosome 11 D11S285. Nucleic Acids Res. 16: 378, 1988. PubMed: 2893342 |