微信小陈
微信小章
产品简介
购买须知
| 产品名称： | C122/U3-T1 C C122/T1, C122/U3-T1 |
|---|---|
| 商品货号： | TS179209 |
| Designations： | C122/U3-T1 C C122/T1, C122/U3-T1 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Vector： | Construct size (kb): 27.60000038146973 |
| Insert： | DNA: genomic Insert lengths(kb): 20.0 Gene product: methionyl-tRNA synthetase MSM1 |
| Insert Size (kb)： | 20.0 |
| Genotype： | MATalpha ura3-1 msm1-1 |
| Mating Type： | alpha |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites separated by (kb) (approximately): SacI- 0.3- KpnI- 1.3- KpnI- 1.0- BamHI- 1.2- XbaI- 0.8- BamHI- 0.4- PstI- 0.4- XbaI- 0.4- BamHI- 0.4- PstI- 0.4- KpnI- 0.5- SacI. Restriction digests of the clone give the following sizes (kb): SalI--27.7; EcoRV--12.8, 4.4, 3.3 (doublet), 1.5, 1.2, 1.0; XbaI--17.0, 5.6, 3.3, 1.7; BamHI--23.1, 2.2, 1.7; SmaI/SalI--13.1, 8.6, 3.4, 0.6. MSM1 is the nuclear gene encoding the mitochondrial methionyl-tRNA synthetase. The smallest region capable of complementing a msm1-1 mutation is defined by a KpnI-PstI fragment of approximately 3 kb from the middle of the insert, and spanning the BamHI and XbaI sites separated by 1.2 kb. The probe, pG72/T1, has been deposited in Escherichia coli RR1 as TS179209 and in Saccharomyces cerevisiae C122/U3 as ATCC 66319. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Tzagoloff A, et al. Characterization of MSM1, the structural gene for yeast mitochondrial methionyl-tRNA synthetase. Eur. J. Biochem. 179: 365-371, 1989. PubMed: 2645139 |