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| 产品名称： | RhC7/pSVL |
|---|---|
| 商品货号： | TS179340 |
| Designations： | RhC7/pSVL |
| Species： | Homo sapiens, human |
| Depositors： | G Vassart |
| Applications： | When cotransfected into CHO cells with pSV2neo, the construct expresses the receptor. |
| Vector： | Construct size (kb): 6.400000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSVL Intact vector size: 4.889 Type of vector: plasmid Vector end: XhoI Vector end: XbaI Cloning sites: XhoI XbaI SmaI XmaI SacI BamHI Polylinker sites: XhoI XbaI SmaI XmaI SacI BamHI Construction: pBR322, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 late replicon: pMB1, SV40 terminator: SV40 late poly(A) Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ADORA1 Genomic copy number: unique Gene name: adenosine A1 receptor Contains complete coding sequence?: Y Chromosome: 1; Localization: 1 q32.1 Tissue: hippocampus Type of DNA: cDNA Insert 5 end: PstI Insert 3 end: XbaI Insert size (kb): 1.5 Cross references: Insert lengths(kb): 1.5 Tissue: hippocampus Gene product: adenosine A1 receptor ADORA1 |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI/XbaI--3.3, 2.3, 1.9; BamHI--6.8, 0.9; EcoRI--5.4, 2.3; HindIII--5.8, 1.35, 0.6; XhoI--7.8. When cotransfected into CHO cells with pSV2neo, the construct expresses the receptor. The construct includes an XhoI/PstI fragment derived from a pBluescript SK+ multiple cloning sequence. The PstI site is at the 5 end of the cDNA. The PstI/XbaI fragment includes 350 bp of 5 untranslated and 220 bp of 3 untranslated sequence. |
| References： | Libert F, et al. Cloning and functional characterization of a human A1 adenosine receptor. Biochem. Biophys. Res. Commun. 187: 919-926, 1992. PubMed: 1530647 Gilbert Vassart, personal communication |