| 产品名称: | pGem3H2.1/HH250 |
|---|---|
| 商品货号: | TS179374 |
| Designations: | pGem3H2.1/HH250 |
| Species: | Mus musculus, mouse |
| Depositors: | FH Ruddle |
| Vector: | Construct size (kb): 3.150000095367432 |
| Insert: | DNA: genomic Insert lengths(kb): 0.25 Tissue: embryo Gene product: homeo box-2 cluster, gene 1 HOX2 |
| Insert Size (kb): | 0.25 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | Restriction digests of the clone give the following sizes (kb): HindIII--3.25; HaeIII--0.64, 0.6, 0.44, 0.26, + smaller fragments; EcoRI/HindIII--3.0, 0.24. Contains all but the first 10 bp of the homeo box. Detects transcripts of 1.7 and 1.9 kb in murine brain and cervical and thoracic spinal column, but not in the lumbar spinal column. pMo4-1, which contains this sequence, detects a human genomic EcoRI fragment of 12 kb and a mouse genomic EcoRI fragment of 2.8 kb. |
| Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References: | Hart CP, et al. Homeo box gene complex on mouse chromosome 11: molecular cloning, expression in embryogenesis, and homology to a human homeo box locus. Cell 43: 9-18, 1985. PubMed: 3000607 Utset MF, et al. Region-specific expression of two mouse homeo box genes. Science 235: 1379-1382, 1987. PubMed: 2881353 |