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| 产品名称： | pTATALUC+ |
|---|---|
| 商品货号： | TS179390 |
| Designations： | pTATALUC+ |
| Depositors： | J Duschl, J Altschmied |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 4.6430001258850100 DESCRIPTION OF VECTOR: Intact vector size: 4.643 Type of vector: plasmid Cloning sites: HindIII PstI SalI BamHI ClaI SmaI KpnI SacI EcoRI Polylinker sites: HindIII PstI SalI BamHI tk-promoter luc+ SV40 poly (A)ClaI SmaI KpnI SacI EcoRI Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): MCS: HindIII...BamHI, ->, 1-36 promoter: truncated TK promoter, 37-131 reporter group: luciferase (luc+), ->, 170-1822 terminator: SV40 late polyadenylation, 1854-2075 MCS: ClaI...EcoRI, 2078-2106 replicon: pMB1, 2519-2519 marker(s): ampR, terminator: SV40 large T-antigen polyadenylation, 4269-4640 Vector: pTATALUC+ (plasmid) Promoters: Promoter truncated TK promoter Marker(s):ampR Construct size (kb): 4.64300012588501 Features: marker(s): ampR promoter: truncated TK promoter replicon: pMB1 reporter group: luciferase (luc+) MCS: ClaI...EcoRI MCS: HindIII...BamHI terminator: SV40 large T-antigen polyadenylation terminator: SV40 late polyadenylation |
| Applications： | reporter construct shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.6; HindIII--4.6; EcoRI--4.6. The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements. The promoters driving luc+ transcription in these two vectors represent different 5 deletions of the tk promoter ending at -32 and -105, respectively. The vector pTATALUC+ carries only the TATA box to avoid interference of the tk-derived CAAT and GC boxes with the regulatory elements of interest. The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements. The vectors pTATALUC+ (TS179390) and ptkLUC+ (ATCC 87632) were designed for the characterization of isolated cis regulatory elements. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214 |
| Related Products： | component of:ATCC 87654 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |