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| 产品名称： | pJEL170 |
|---|---|
| 商品货号： | TS179443 |
| Designations： | pJEL170 |
| Depositors： | P Valentin-Hansen |
| Biosafety Level： | 1 |
| Host： | Escherichia colixa0K-12 CSH50 |
| Vector Information： | Size (kb): 12.50
Vector: pJEL170 (plasmid)
Construction: pJEL126, pJEL154
Marker(s): ampR
Construct size (kb): 12.5
Features: insert detection: lacZ marker(s): ampR replicon: R1 repressor gene: cI857 |
| Applications： | Promoter-cloning vector Vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--13.0; EcoRI--13.0. Below 37C, there is one copy per genome equivalent stabilized by the parB locus. At 42C replication is uncontrolled because induction of the cI857-regulated PR promoter permits expression of the copB, copA, and repA genes. A promoter-cloning vector using increased expression of beta-galactosidase as the reporter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 35°C |
| References： | Larsen JE, et al. Analysis of the terminator region after the deoCABD operon of Escherichia coli K-12 a new class of single copy number operon-fusion vectors. Nucleic Acids Res. 15: 5125-5140, 1987. PubMed: 3299264 |
| Shipped： | freeze-dried |