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微信小章| 产品名称： | UPCI:SCC090 |
|---|---|
| 商品货号： | TS179609 |
| Organism： | Homo sapiens, human |
| Tissue： | Derived from metastatic site: tongue |
| Cell Type： | squamous cell |
| Product Format： | frozen 1.0 mL |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain human papillomavirus (HPV) DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma; grade 3; pathological stage: T2N0 |
| Age： | 46 |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line represents an important resource for investigation into the tumor biology and the study of genetic abnormalities associated with the development and/or progression of HNSCC. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The surgically excised tumor was minced and distributed into flasks. When growth of fibroblasts obstructed the growth of the squamous cells, the fibroblasts were removed by trypsinization. The remaining squamous cells were grown and passaged. |
| Clinical Data： | 46 years
Caucasian
male
The donor smoked tobacco, drank alcoholic beverages, and died of his disease |
| Antigen Expression： | CK 19+; Keratin IHC AE1+; Keratin IHC AE3+; CAM 5.2+ |
| Oncogene： | amplification of chromosomal band 11q13, not expressed mutations of TP53 codon, not expressed |
| Comments： | UPCI:SCC090 is from a squamous cell carcinoma of the base of the tongue. These cells are positive for Human Papilloma Virus (HPV). They have no TP53 mutations as assayed by sequencing the 5-8 exons of TP53. UPCI:SCC090 showed no amplification of chromosomal band 11q13 using FISH. The patient had a recurrence about 1 year later sited at the hypopharynx. It is ATCC® No. CRL-3240 UPCI:SCC152™. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium:
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| Seeding Density： | 4 x 104 viable cells/cm2 |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: 2 to 3 times a week |
| Volume： | 1.0 mL |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D5S818: 11, 12 D7S820: 9, 10 D13S317: 11 D16S539: 12,13 TH01: 7 TPOX: xa08 vWA: 17 |
| Name of Depositor： | Susanne M. Gollin, University of Pittsburgh |
| Year of Origin： | September 1994 |
| References： | White JS, et al. The influence of clinical and demographic risk factors in the establishment of head and neck squamous cell carcinoma cell lines. Oral Oncol. 43:701-712, 2007. PubMed: 17112776 Martin CL, et al. Chromosomal imbalances in oral squamous cell carcinoma: examination of 31 cell lines and review of the literature. Oral Oncol. 44:369-382, 2008. PubMed: 17681875 Ragin C, et al. Mapping and analysis of HPV16 integration sites in a head and neck cancer cell line. Int. J. Cancer 110:701-709, 2004. PubMed: 15146560 |