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| 产品名称： | pGIBS-TRP phagemid in E. coli |
|---|---|
| 商品货号： | TS179622 |
| Designations： | pGIBS-TRP phagemid in E. coli |
| GenBank Number： | K01391 |
| Species： | Bacillus subtilis (Ehrenberg) Cohn |
| Depositors： | M Follettie |
| Applications： | For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). |
| Vector： | Construct information: Total size of construct (kb): 5.4 Marker: ampR Promoter giving the sense strand: T3 Promoter giving the antisense strand: T7 Excise insert: XhoI+NotI Vector information: Vector: pBluescriptII KS- Vector type: phagemid Vector size (kb): 2.961 Markers: ampR Vector end (5’): XhoI Vector end (3’): NotI |
| Insert： | Insert size (kb): 2.5 Gene: trpCDEF DNA: genomic DNA Source: Bacillus subtilis Genbank accession: K01391 (Nucleotides 1-2518 of the insert correspond to nucleotides 1883-4400 of K01391.) Insert end (5’): XhoI linker Insert end (3’): BamHI/polyA/NotI linker |
| Insert Size (kb)： | 2.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze dried E. coli containing the plasmid |
| Comments： | For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). The insert is a PCR amplified B. subtilis trp gene fragment (Genbank KO1391 from nt 1883 - 4400), with an added artificial polyA tail. This insert was then cloned into the XhoI->NotI sites of pBluescript II KS+. The insert contains the following restriction sites (approximate kb from the 5 end): BglII--1.25; PstI--2.06; XbaI--2.25. Restriction digests of the clone give the following sizes (kb): NotI--5.6; NotI/XhoI--3.0, 2.8; XhoI--5.6. |