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| 产品名称： | HHCMD33 D17S2061, UTR-9804 |
|---|---|
| 商品货号： | TS179700 |
| Designations： | HHCMD33 D17S2061, UTR-9804 |
| GenBank Number： | M34175 M78004 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ADTB* Gene name: adaptin, beta Contains complete coding sequence?: N Chromosome: 17; Localization: 17, cR Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 2.3 Cross references: DNA Seq. Acc.: M34175; EST: EST01589 Nucleotides 1-207, 207-319 of the insert correspond to nucleotides 835-1041, 1040-1152 of M34175 with 99% identity. DNA Seq. Acc.: M78004; EST: EST01589; Primer: M13 Forward; THC id: THC125091 Insert lengths(kb): 2.299999952316284 Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205) Gene product: adaptin, beta ADTB* |
| Insert Size (kb)： | 2.300 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.4, 0.9. The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs. Based on the size of the insert and the alignment with GenBank accession M34175 (5701 nt, CDS178-2991), this clone probably contains part of the coding sequence and part of the 3 untranslated region. |
| References： | Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 |