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| 产品名称： | lambdaNK1224 NK1224, lambdaTn10LUK |
|---|---|
| 商品货号： | TS179749 |
| Designations： | lambdaNK1224 NK1224, lambdaTn10LUK |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | N Kleckner |
| Applications： | In hosts lacking a nonsense suppressor, lysogenization and lytic growth of the phage are prevented by the cI- Int- and P- phenotypes of the bacteriophage. Recovery of transpositions into plasmid replicons are obtained by transforming plasmid DNA from kanR colonies into a lambda resistant host such as E. coli NK8017 (ATCC 47057). |
| Vector： | Construct size (kb): 50.0 |
| Insert： | DNA: genomic Insert lengths(kb): 9.399999618530273 Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5-phosphate decarboxylase, orotate phosphoribosyltransferase 1) URA3 |
| Insert Size (kb)： | 9.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--23.0, 19.4, 9.4, 5.6, 3.8, 3.0; BamHI--27.0, 17.0, 11.0; PvuII--22.5, 15.0, 9.4, 6.0, 3.3, 1.7, 1.1, 0.7, 0.5. In hosts lacking a nonsense suppressor, lysogenization and lytic growth of the phage are prevented by the cI- Int- and P- phenotypes of the bacteriophage. Recovery of transpositions into plasmid replicons are obtained by transforming plasmid DNA from kanR colonies into a lambda resistant host such as E. coli NK8017 (ATCC 47057). For insertional mutagenesis of bacterial or yeast genes carried in plasmid vectors. Contains the mini-Tn10-LUK insert that consists of the following features: IS10R - nt 63 - promoterless lacZ element starting at codon 8 - nt 3140 - URA3 - nt 4320 - kanR - nt 6023 - IS10L - nt 6090. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Huisman O, et al. A Tn10-lacZ-kanR-URA3 gene fusion transposon for insertion mutagenesis and fusion analysis of yeast and bacterial genes. Genetics 116: 191-199, 1987. PubMed: 3038670 Kleckner N, et al. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204: 139-180, 1991. PubMed: 1658561 Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 |