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| 产品名称： | 5HT1B |
|---|---|
| 商品货号： | TS179803 |
| Designations： | 5HT1B |
| Species： | Mus musculus, mouse |
| Depositors： | MJ Bienkowski, SA Veldman |
| Applications： | in another host, produces protein 5-hydroxytryptamine (serotonin) receptor 1b |
| Vector： | Construct size (kb): 9.399999618530273 |
| Insert： | DNA: genomic Insert lengths(kb): 1.850000023841858 Gene product: 5-hydroxytryptamine (serotonin) receptor 1b Htr1b Target Gene: 5-hydroxytryptamine (serotonin) receptor 1b |
| Insert Size (kb)： | 1.850 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--8.8, 0.6; EcoRI--6.5, 2.3, 0.86; HindIII--7.6, 1.9; PstI--5.6, 2.1, 1.3, 0.6; XhoI--9.4. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--1.25, 1.77; BglI--0.74; EcoRV--0.86; KpnI--0.54; PstI--0.04, 0.57; SmaI--0.06, 0.21, 1.27, 1.30. Includes approximately 482 bp of 5 untranslated sequence, the complete coding sequence and approximately 210 bp of 3 untranslated sequence. The insert is in the forward orientation relative to the CMV promoter. The order of the major features of the plasmid is: ampR - EcoRI - SV40 ori - SV40 promoter - XhoI - hygR - tk polyadenylation - TaqI - SV40 polyadenylation - EcoRI - CMV promoter - HindIII - insert - HindIII - SalI - bGH polyadenylation - PvuI. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Sarah A Veldman, personal communication |