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微信小章| 产品名称： | B-MEKDD 116 |
|---|---|
| 商品货号： | TS179807 |
| Organism： | Mus musculus, mouse |
| Tissue： | breast epithelium (mammary gland) |
| Cell Type： | epithelial cell |
| Product Format： | frozen |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | This cell line stably expresses constitutively activated form of MEK1, and can be used in MEK-MAPK pathway studies. This cell line is tumorigenic, and can be used to build a breast cancer in vivo model. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | B-MEKDD 116 was produced by transfecting parental EpH4, a mouse mammary epithelial cell line, with an expression vector containing Glu-Glu epitope-tagged phosphorylation site MEK1 mutant (MEKDD).xa0 After selection in complete medium supplemented with 1 mg/mL G418 for 14 days, individual clones were isolated and expanded. |
| Oncogene： | Constitutively activated form of MEK1, expressed |
| Genes Expressed： | Glu-Glu tagged phosphorylation site MEK1 mutant (MEKDD), verified at ATCC |
| Cellular Products： | stably expresses Glu-Glu tagged MEKDD, which is constitutively activated form of MEK1 (Asp218/Asp222 MEK1 phosphorylation site mutant) |
| Tumorigenic： | Yes |
| Comments： | B-MEKDD 116 was produced by transfecting parental EpH4, a mouse mammary epithelial cell line, with an expression vector containing Glu-Glu epitope-tagged phosphorylation site MEK1 mutant (MEKDD).xa0 After selection in complete medium supplemented with 1 mg/mL G418 for 14 days, individual clones were isolated and expanded. Activation of MEK1 is mediated through phosphorylation of Ser218 and Ser222 by members of the Raf family of kinases.xa0This cell line stably expresses constitutively activated form of MEK1 (Asp218/Asp222 MEK1 phosphorylation site mutant), and can be used in MEK-MAPK pathway studies. This cell line is tumorigenic, and can be used to build a breast cancer in vivoxa0model. There are four additional related cell lines: EpH 1424 cell line (ATCC CRL-3071), EpH4 1424.1 cell line (ATCC CRL-3209), EpH4 1424.2 cell line (ATCC CRL-3210), and EpH4-Ev cell line (ATCC CRL-3063). The B-MEKDD 116 cell line produces the constitutively activated MEK1 mutant MEKDD which has been tagged with Glu-Glu, verified at ATCC. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated DMEM Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 10% Bovine Calf Serum and 200 mcg/mL G418
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:6 to 1:12 is recommended. Medium renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: complete growth medium supplemented with an additional 10% bovine calf serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | P Leder |
| References： | Pinkas J, et al. MEK1 signaling mediates transformation and metastasis of EpH4 mammary epithelial cells independent of an epithelial to mesenchymal transition. Cancer Res. 62(16): 4781-90. PubMed: 12183438 Pinkas J, et al. Bcl-2-mediated cell survival promotes metastasis of EpH4 betaMEKDD mammary epithelial cells. Mol. Cancer Res. 2(10): 551-6, 2004. PubMed: 15498929 |