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| 产品名称： | pC4ro3 |
|---|---|
| 商品货号： | TS179836 |
| Designations： | pC4ro3 |
| Depositors： | TJ Lockett |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.8000001907348600 Vector: pC4ro3 (plasmid) Construction: pro1, Carnegie 4, pSL1180 MCS Marker(s):ampR Construct size (kb): 10.80000019073486 Features: marker(s): ampR replicon: pMB1 |
| Applications： | contains sequence rough expression vector in another host, produces protein rough reporter construct shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--8.6, 2.2; HindIII--7.7, 3.1; PstI--10.8. The SphI/HindIII fragment corresponding to the rough gene contains the following restriction sites (kb from the 5 end): BamHI--3.02; BglII--5.39; EcoRI--0.91, 4.91; SacI--0.57, 4.19, 5.86; SalI--1.80, 3.91; SmaI--4.43. Vector containing a non-autonomous P-element and incorporating the rough gene (ro) as a dominant marker for P-element mediated gene transfer into the germ line of Drosophila melanogaster. The order of the major features in this plasmid is: HindIII - P-element/Carnegie 4/P-element - XhoI/MCS/SphI - ro gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Lockett TJ, et al. The rough (ro+) gene as a dominant P-element marker in germ line transformation of Drosophila melanogaster. Gene 114: 187-193, 1992. PubMed: 1601302 Trevor J Lockett, personal communication |
| Shipped： | freeze-dried |