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| 产品名称： | p414 ADH |
|---|---|
| 商品货号： | TS179974 |
| Designations： | p414 ADH |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.514 DESCRIPTION OF VECTOR: Intact vector size: 6.514 Type of vector: plasmid Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI Other unique sites: SacI Construction: pRS414 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): TRP1, ->, 200-1213 terminator: CYC1, ->, 1895-2155 MCS: XhoI...XbaI, ->, 2155-2219 promoter for expression: ADH, marker(s): ampR, replicon: CEN6/ARSH4, ->, 6001-6514 Vector: p414 ADH (plasmid) Promoters: Promoter for expression ADH Construction: pRS414 Marker(s):TRP1,ampR Construct size (kb): 6.514 Features: marker(s): TRP1 marker(s): ampR promoter for expression: ADH replicon: CEN6/ARSH4 MCS: XhoI...XbaI terminator: CYC1 |
| Applications： | YX-type (expression) shuttle vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--6.6; XhoI--6.6; SacI/XbaI--3.2, 1.8, 1.5. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources. Low copy number shuttle expression vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | component of:ATCC 87669 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |