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产品简介
购买须知
| 产品名称： | pLOX plasmid in E. coli |
|---|---|
| 商品货号： | TS179976 |
| Designations： | pLOX plasmid in E. coli |
| Depositors： | M Kyba |
| Biosafety Level： | 1 |
| Vector Information： | Cloning sites: EcoRI,KpnI,MluI,SalI,SmaI,XbaI,XhoI Size (kb): 5.2000000000000000 Vector: pLOX plasmid in E. coli Promoters: Promoter for expression PGK Marker(s):AmpR Features: reporter group: EGFP transcription terminator: SV40 polyA |
| Comments： | A lox-targeting vector.
The stuffer neo- EGFP is bounded by multiple cloning sites upstream and downstream.The 5 MCS consists of XhoI, EcoRI , MluI and KpnI. The 3 MCS consists of NotI, XbaI, SalI, SmaI and another NotI site.
The order of the features of this plasmid are: PGK (phosphoglycerokinase) promoter - lox - 5 MCS - neo - EGFP - 3 MCS - SV40 polyA - f1 ori - ampR - pUC ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Kyba M, et al. HoxB4 confers definitive lymphoid-myeloid engrafment potential on embryonic stem cell and yolk sac hematopoietic progenitors. Cell 109: 29-37, 2002. Kyba M, et al. Development of hematopoietic repopulating cells from embryonic stem cells. Methods Enzymol. 365: 114-129, 2003. |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen E. coli containing the plasmid |