微信小陈
微信小章| 产品名称： | Piscirickettsia salmonis Fryer et al. |
|---|---|
| 商品货号： | TS180056 |
| Classification： | Thiotrichales, Piscirickettsiaceae, Piscirickettsia |
| Agent： | Piscirickettsia salmonis Fryer et al. |
| Strain： | LF-89 |
| Biosafety Level： | 2 This is an exotic fish pathogen and caution should be exercised when working with this agent to avoid introducing it into environments where it has not been previously reported to exist.
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | clinical specimen - animal Chile Isolation date: October, 1989 |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | This organism is a gram-negative, non-motile, pleomorphic 0.5 to 1.5 micometer diameter coccoid. |
| Effect on Host： | Cytopathic effects in tissue culture (small refractile rounding and clumping) CPE; small refractile rounding and clumping |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10 mM HEPES, 90%; fetal bovine serum, 10%
|
| Recommended Host： | Recommended Host:xa0 CHSE-214 (ATCC® CRL-1681TM) |
| Growth Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 15°C to 18°C Duration: 6 to 14 days; For best results host cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent). |
| Name of Depositor： | JL Fryer, CN Lannan |
| Special Collection： | ATCC |
| Source： | clinical specimen - animal Chile Isolation date: October, 1989 |
| Geographical Isolation： | South America |
| Year of Origin： | October, 1989 |
| References： | Fryer JL, Mauel MJ. The rickettsia: an emerging group of pathogens in fish. Emerg. Infect. Dis. 3: 137-144, 1997. PubMed: 9204294 Fryer JL, et al. Isolation of a rickettsiales-like organism from diseased Coho salmon (Oncorhynchus kisutch) in Chile. Fish Pathol. 25: 107-114, 1990. VR-1361 is an exotic fish pathogen and caution should be exercised when working with this agent to avoid introducing it into environments where it has not been previously reported to exist. Fryer JL, et al. Piscirickettsia salmonis gen. nov., sp. nov., the causative agent of an epizootic disease in salmonid fishes. Int. J. Syst. Bacteriol. 42: 120-126, 1992. PubMed: 1371057 Isolated by J. L. Fryer and C. N. Lannan from the kidney of a Coho salmon (Oncorhynchus kisutch) displaying lethargy, anemia, swollen kidney, splenomegaly, darkened coloration and rare hepatic lesions. small refractile rounding and clumping |