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| 产品名称： | smart2 |
|---|---|
| 商品货号： | TS180157 |
| Designations： | smart2 |
| Depositors： | JW Raff |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.6000003814697270 Vector: smart2 (cosmid) Promoters: Promoter T7 Construction: Lorist2, cosPneo Marker(s):G418R,tetR Construct size (kb): 9.600000381469727 Features: marker(s): tetR, G418R promoter: SP6, T7 replicon: lambda terminator: none enhancer: none |
| Applications： | expression vector integrating vector shuttle vector vector for constructing cDNA libraries vector permitting RNA synthesis in vitro vector permitting positive selection for integration |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--9.6; SalI--9.6. G418 resistance (neo) is expressed under the control of the Drosophila hsp70 promoter. There are no pBR322 sequences in the vector. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Cosmid vector for chromosome walking and P-element-mediated germline transformation in Drosophila. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Speek M, et al. Smart2, a cosmid vector with a phage lambda origin for both systematic chromosome walking and P-element-mediated gene transfer in drosophila. Gene 64: 173-177, 1988. PubMed: 2969350 |
| Shipped： | freeze-dried |