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| 产品名称： | pBSL159 |
|---|---|
| 商品货号： | TS180186 |
| Designations： | pBSL159 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.1440000534057620 Vector: pBSL159 (plasmid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pSU18 Marker(s):ampR,cmlR Construct size (kb): 4.144000053405762 Features: marker(s): cmlR promoter for in vitro transcription: T7 (phi10) replicon: p15A MCS: KpnI...SacI MluI MCS: MluI SacI...KpnI coding sequence: bla (ampR) |
| Applications： | contains easily purifiable cassette(s) for construction omega - Ap |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.1, 2.0; EcoRI--2.1 (doublet); KpnI--2.15, 2.0; MluI--2.2, 2.0; SstI--2.15, 2.0. Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Ap element flanked by multiple cloning sites duplicated in inverted orientation. The PstI and HincII sites in the bla gene have been removed. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718 |
| Shipped： | freeze-dried |