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| 产品名称： | pSP65CS |
|---|---|
| 商品货号： | TS180219 |
| Designations： | pSP65CS |
| Depositors： | RL Eckert |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.0799999237060550 Vector: pSP65CS (plasmid) Promoters: Promoter SP6 Construction: pGV403, pSP65 Marker(s):ampR Construct size (kb): 3.079999923706055 Features: marker(s): ampR promoter: SP6 replicon: pMB1 terminator: none enhancer: none |
| Applications： | vector permitting RNA synthesis in vitro |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.2; BamHI--3.2; EcoRI--3.2. High copy number plasmid vector for directional sequencing of fragments with a sticky end corresponding to one the polylinker sites or bidirectional sequencing of blunt-ended fragments. Ideal for sequencing nested sets of deletions. pSP64CS (ATCC 37635) and pSP65CS (TS180219) differ only in the orientation of the polylinker relative to the SP6 promoter. The 80 bp EcoRI/HindIII fragment (ends filled) of pGV403 with sequencing configuration was cloned in the SmaI site of modified pSP65 (Tth111 site removed). SP6 promoter is next to EcoRI and directs transcription across the MCS toward HindIII. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | . Current protocols in molecular biology. New York: Greene Pub. Associates; 1987. . Current protocols in molecular biology. New York: Greene Pub. Associates; 1987. Eckert RL. New vectors for rapid squencing of DNA fragments by chemical degradation. Gene 51: 247-254, 1987. PubMed: 3596245 |
| Shipped： | freeze-dried |